ABSTRACT
Bacillus cereus causes two types of gastrointestinal diseases: emesis and diarrhea. It produces one emetic toxin and nine different enterotoxins. In March 2008, eight of a family became sick after eating slices of raw fish. We isolated emetic toxin producing B. cereus from the stools of 6 patients and 2 subclincal humans. In this study, the presence of enterotoxin genes, such as those of haemolysin BL (Hbl), nonhemolytic enterotoxin (Nhe), B. cereus enterotoxin T (BceT), enterotoxin FM (EntFM), cytotoxin K (cytK) and cereulide were assayed by polymerase chain reaction (PCR) methods. Their enterotoxin activities were assayed using the BCET- RPLA, Tecra ELISA kit and Hep-2 vacuole activity. Bacterial isolates were subtyped by pulsed-field gel electrophoresis (PFGE). This study demonstrates the emetic toxin-producing stains of B. cereus in clinical specimens, for the first time in the Republic of Korea.
Subject(s)
Humans , Bacillus , Bacillus cereus , Coloring Agents , Depsipeptides , Diarrhea , Eating , Electrophoresis, Gel, Pulsed-Field , Enterotoxins , Enzyme-Linked Immunosorbent Assay , Polymerase Chain Reaction , Republic of Korea , Vacuoles , VomitingABSTRACT
No Abstract Available.
Subject(s)
Vibrio parahaemolyticus , Vibrio , Virulence Factors , VirulenceABSTRACT
No Abstract Available.
Subject(s)
Methicillin Resistance , Methicillin , Methicillin-Resistant Staphylococcus aureus , Staphylococcus aureus , StaphylococcusABSTRACT
No Abstract Available.
Subject(s)
Vibrio parahaemolyticus , Vibrio , Virulence Factors , VirulenceABSTRACT
No Abstract Available.
Subject(s)
Methicillin Resistance , Methicillin , Methicillin-Resistant Staphylococcus aureus , Staphylococcus aureus , StaphylococcusABSTRACT
Sixteen strains of LT-producing enterotoxigenic E. coli 0128 which were isolated from diarrheal patient's stool in Pusan University Hospital, were serotyped and analyzed for plasmid DNA profile, MRHA of human blood cells, and also tested for possession of LT, ST, aggA, EAST1 genes by the PCR method and analyzed the RAPD pattern. Screening sensitivity for ETEC by salting out test was 87.5%. These data suggest that hydrophobicity test using salting out is rapid, inexpensive, and simple screening test for ETEC. CFAs were identified in 87.5% of strains; 43.75% the strains harbored CFA/I, 43.75% CFA/II, and 12.5% expressed none of these CFAs. For plasmid profiles, 12 strains had 60 MDa plasmid and several smaller plasmids. The strains showed 5 types of plasmid profiles. By PCR, LT gene but not ST gene was detected from all of the 16 strains EAST1 gene was detected from 14 strains. Ten strains could be differentiated to 3 patterns by chromosomal DNA fingerprint. The chromosomal DNA fingerprinting is considered very useful for the epidemiological study.